Bulk RNA-sequencing of FACS-sorted kidney glomerular cell types from BTBR ob/ob mice and glomeruli isolated from ex vivo perfused kidneys

To investigate the gene expression levels of major glomerular cell types in BTBR ob/ob mice and in glomeruli under hyperfiltration Bulk RNA-sequencing of glomerular cell types from BTBR ob/ob and BTBR +/+ mice at three time points (6/12/18 weeks). The glomeruli were isolated from ex vivo perfused kidney (hyperfiltration) and unperfused kidney (control) from the mouse. Overall design: Comparative gene expression profiling analysis of RNA-seq data (BTBR ob/ob vs. BTBR +/+ in each glomerular cell type). Comparative gene expression profiling analysis of RNA-seq data for perfused vs unperfused glomeruli.