Supporting Information S1 - Function of the Active Site Lysine Autoacetylation in Tip60 Catalysis

Figures S1 through S11 show the tandem mass spectrometric data for the acetylated lysine residues in Tip60 protein. Figure S1. LC-MS/MS analysis of the AA(67-80) peptide sequence showing Tip60 is acetylated at lysine 76. Figure S2. LC-MS/MS analysis of the AA(77-104) peptide sequence showing Tip60 is acetylated at lysine 80. Figure S3. LC-MS/MS analysis of the AA(98-120) peptide sequence showing Tip60 is acetylated at lysine 104. Figure S4. LC-MS/MS analysis of the AA(94-115) peptide sequence showing Tip60 is acetylated at lysine 104. Figure S5. LC-MS/MS analysis of the AA(94-124) peptide sequence showing Tip60 is acetylated at lysine 104. Figure S6. LC-MS/MS analysis of the AA(150-177) peptide sequence showing Tip60 is acetylated at lysine 150. Figure S7. LC-MS/MS analysis of the AA(180-188) peptide sequence showing Tip60 is acetylated at lysine 187. Figure S8. LC-MS/MS analysis of the AA(324-347) peptide sequence showing Tip60 is acetylated at lysine 327. Figure S9. LC-MS/MS analysis of the AA(320-334) peptide sequence showing Tip60 is acetylated at lysine 327. Figure S10. LC-MS/MS analysis of the AA(320-340) peptide sequence showing Tip60 is acetylated at lysine 327. Figure S11. LC-MS/MS analysis of the AA(380-398) peptide sequence showing Tip60 is acetylated at lysine 383.
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