Transcriptome analysis of valosin-containing protein effect on cardiac hypertrophy and dysfunction

This sstudy was designed to investigate the gene netweork regulated by valosin-containing protein (VCP), an ATPase-associated protein, whic acts as a novel regulator against the cardiac stress of pressure overload. We performed transcriptome analyses to compare cardiac-specific VCP overexpression transgenic (TG) mice and wild-type (WT) mice in a series of pathological models. The left ventricular (LV) tissues were collected from both WT and VCP TG mice at 2w post TAC and at 2w post sham operations. mRNA was extracted and sequenced to generate gene expression profiles. Multiple comparisons were performed between VCP TG and WT mice at two treatment conditions: sham and 2w post TAC. Our studies revealed novel molecular mechanisms by which VCP protects heart against pressure overload-induced cardiac hypertrophy and the transition to the heart failure. Overall design: Left ventricular tissue of WT and VCP TG mice at 2w post sham operations (n=4 for both) and at 2w (n=4 for WT, n=3 for VCP TG) post TAC were collected for transcriptome analysis. Left ventricular tissue of WT and VCP TG mice at 5w (n=4 for WT, n=3 for VCP TG) post TAC were collected for transcriptome analysis.