Lung cancer images

The slides of lung cancer tissues were stained with hematoxylin-eosin (HE) in standard histopathological procedures. Formalin-fixed and paraffin-embedded tissue sections were cut into 4 µm thick slices, and were stained using a Tissue-Tek DRS 2000E-D2 Slide Stainer (Sakura Finetek Japan) according to the manufacturer’s instructions. Using the AxioVision SE64Rel.4.9.1.1 (Carl Zeiss Meditec AG, Germany) software, images were captured with an Axio Imager Z.1 (Carl Zeiss Meditec AG, Germany) microscope equipped with an EC Plan-NEOFLUOAR 20x/0.5NA lens.

该数据集的肺癌组织玻片采用标准组织病理学流程完成苏木精-伊红（hematoxylin-eosin，HE）染色。具体实验流程为：将福尔马林固定石蜡包埋的组织切片切割为4微米厚的薄片，使用Tissue-Tek DRS 2000E-D2玻片染色机（Sakura Finetek Japan，樱花芬泰克日本公司）依照制造商提供的操作说明进行染色。随后，通过搭载EC Plan-NEOFLUOAR 20×/0.5NA物镜的Axio Imager Z.1型显微镜（卡尔蔡司医疗技术股份公司，Carl Zeiss Meditec AG，德国），配合AxioVision SE64Rel.4.9.1.1（卡尔蔡司医疗技术股份公司，Carl Zeiss Meditec AG，德国）软件完成图像采集。