Hydrogen uptake activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge during METEOR cruise M78/2

Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogen uptake (hydrogenase) activity by means of an activity-based screen. The hydrogen uptake activity of positively screened clones was determined by means of a spectrophotometric assay and the DNA-sequences of the respective fosmid inserts were determined by PacBio Sequencing. The dataset contain hydrogen-uptake activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields.