Effect of deletion of Irf3, Irf7 or Irf9 on gene expression in sgScd2 Th1 cells.. Effect of deletion of Irf3, Irf7 or Irf9 on gene expression in sgScd2 Th1 cells.

To investigate the role of the IRF family in the regulation of interferon stimulated genes, we conducted CRISPR/Cas9 mediated genome editing of Irf3, Irf7 or Irf9 in sgScd2 Th1 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of control, sgScd2, sgScd2/sgIrf3, sgScd2/sgIrf7 or sgScd2/sgIrf9 Th1 cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Th1 cells and its KO derivatives (sgScd2, sgScd2/sgIrf3, sgScd2/sgIrf7 and sgScd2/sgIrf9 Th1 cells).