Estrogen receptor ChIP-seq in response to mTOR inhibition. Homo sapiens

We have used ChIP-seq to profile binding of the estrogen receptor (ER) to chromatin in response to two mTOR inhibitors, i.e. everolimus (RAD001) and vistusertib (AZD2014). Two hours of treatment with these inhibitors significantly affected mTOR signaling, but surprisingly did not affect binding of ER to chromatin. This suggests that these mTOR inhibitors work through largely ER-independent mechanisms. Overall design: Estrogen receptor ChIP-seq was performed on chromatin from asynchronous MCF7 cells in full estrogenic media treated for 2 hours with vehicle, everolimus, or vistusertib.