Sg SCS suppresses inflammation and oral microbiome

To investigate the properties of Sg spent culture supernatant (SCS) on the proliferation of periodontal pathogens and the expression of proinflammatory cytokines by human macrophages, epithelial cells, and gingival fibroblasts. Overall design: Sg SCS was collected from Sg cultures grown in DMEM medium and added to the growth media of representative health and disease related oral species: Sg, Streptococcus sanguinis (Ss), Streptococcus mitis (Sm), Streptococcus oralis (So), Pg, Tanneralla forsythia (Tf), and Treponema denticola (Td). The Sg SCS was also tested for its ability to regulate expression of proinflammatory cytokines by human macrophages, epithelial cells, and gingival fibroblasts upon stimulation with Pg-derived lipopolysaccharide (Pg-LPS).