Non-enzymatic SETD1A activity drives breast cancer cell proliferation via cyclin K. (RNA-seq)

Breast cancer is the most common cancer in women worldwide. SETD1A, a histone H3 lysine-4 methyltransferase, is associated with poor prognosis in breast cancer. While both its enzymatic and non-enzymatic functions have been implicated in cancer progression, the specific role of SETD1A in breast cancer remains unclear. This study aimed to elucidate the molecular mechanisms underlying SETD1A dependency in breast cancer. Overall design: Examination of gene expression in sgRNA-expressing iCas9 KPL-1 cells on day 7 of doxycycline treatment (sgEmpty and sgSETD1A) Tiling screening for functional domains of SETD1A in iCas9 KPL-1 cells (sgKMT2pool)