A Clock-Driven Neural Network Critical for Arousal
收藏NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE146166
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Purpose: scRNA-Seq was performed to profile hypothalamic mWake+ cells of male at 7 weeks old The hypothalamus of mWake-tdTomato-Cre mice (male, 7 weeks old) were dissociated using modified Act-Seq protocol and flow-sorted to enrich tdTomato+ cells. A small aliquot of flow-sorted cells were labelled with DAPI and counted under the microscope (99% tdTomato+ cells). Cells were loaded into V3.0 10x Genomics Chromium Single Cell system, and 3 libraries (triplicates) were sequenced on Illumina NextSeq 500 with ~150 million reads per library. Sequencing results were processed with 10x Genomics pipeline. Seurat V3 was used in conjunction with Monocle3 to perform downstream analysis.
创建时间：
2023-10-25
