Differential modulation of cell cycle-related transcripts at late seed stages is the key determinant of seed size difference in Brassica juncea varieties, EH2 and Pusajaikisan

We report a comparative transcriptomic analysis from three developing stages of seeds of two cultivars; small-seeded Early Heera2 (EH2) and bold-seeded Pusajaikisan (PJK) in Brassica juncea. Sequencing of eighteen libraries (three biological replicates from each stage) yielded >6.92 billion paired-end reads (one replicate of 30D PJK sample was later removed from further analysis). A total of 112550 transcripts were found to be expressed. Differential expression analysis revealed critical candidates regulating variety-specific seed size difference in B. juncea.