Visceral adipose tissue contributes to improved insulin resistance after DJB via growth hormone-adiponectin axis

To explore the potential mechanism of duodeno-jejunal bypass (DJB) in improving insulin resistance in diabetic rats, we suggest that adipose tissue is involved in the regulation. To investigate genetic changes in adipose tissue after duodeno-jejunal bypass, we performed RNA-seq analysis to establish the relationship between growth hormone, adiponectin, and insulin sensitivity by measuring changes in growth hormone pathways, including downstream JAK2 and MAPK pathways, and investigated non-PPAR signaling pathways. This study deepens our understanding of the complex mechanism by which DJB affects insulin resistance. This meticulous exploration fills an important gap in the literature and may pave the way for more targeted and effective diabetes management interventions. Overall design: In order to study the effect of duodenal-jejunal bypass on adipocytes, we took samples from mesentery and groin of T2DM rats and post-DJB rats, representing visceral fat and subcutaneous fat, respectively, and analyzed the difference of gene expression in adipose tissues of different parts by RNA-seq, analyzed the changes of fat function after duodenal-jejunalbypass, and explored the potential mechanism of fat cell function changes.