RNA-Seq in porcine ovaries treated with recombinant pig follicle-stimulating hormone (rpFSH)

To identify the DE mRNA and lncRNA between the recombinant pig follicle-stimulating hormone traetment group and control grooup. Ten gilts with the same genetic background were divided into 2 groups (reFSH treatment group and control group) with 5 gilts in each group. All the experiment gilts were born in the same day, raised in the same breeding environment, and fed by the diet according to the Nutrient Requirements of Swine Eleventh Revised Edition 2012. At 210 days of age, altrenogest was administered uniformly for 18 consecutive days, and each pig was fed 20 mg per day. After 18 days, all the pigs were stopped feeding altrenogest for 2 days. The guilts of rpFSH treatment group were injected 1000 IU per pig, and the control group were injected the same volume of normal saline. The ovary samples of the selected pigs were collected at 234 days of age after removing surface follicles and immediately frozen in liquid nitrogen to isolate RNA.