Samples (2-10 mg) were extracted with 2:5:2 CDCl3:CD3OD:H2O, ground, and centrifuged to remove particulate. Extracts (10 uL) were analyzed with HILIC-LC-MS using an Agilent Poroshell 120 HILIC-Z co
Pooled QC and process blanks were processed in Agilent Profinder (B.08 SP3, Agilent, Santa Clara, CA) recursive batch feature extraction, with a retention time window from 1.5 to 13.5 min, noise cutof
Metabolites with RSD < 30% in pooled QCs were removed, yielding 365 metabolites in positive mode and 414 in negative mode. Metabolite intensities were normalized by sample weight and total ion coun
Samples (2-10 mg) were extracted with 2:5:2 CDCl3:CD3OD:H2O, ground, and centrifuged to remove particulate. Extracts (10 uL) were analyzed with HILIC-LC-MS using an Agilent Poroshell 120 HILIC-Z co
Samples (2-10 mg) were extracted with 2:5:2 CDCl3:CD3OD:H2O, ground, and centrifuged to remove particulate. Extracts (10 uL) were analyzed with HILIC-LC-MS using an Agilent Poroshell 120 HILIC-Z co