Glycosyltransferases， Mutants and Their Applications
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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The present invention relates to glycosyltransferases， mutants and their applications. Specifically， in the presence of glycosyltransferase， the glycosyl group of the glycosyl group donor is transferred to the hydroxyl group of the C-3 region of the tetracyclic triterpenoid compound; Forming a glycosylated tetracyclictriterpenoid-based compound; Wherein the glycosyltransferase is a glycosyltransferase represented by SEQ ID NO .: 4 or a derivative polypeptide thereof; Or a glycosyltransferase or a derivative polypeptide thereof represented by SEQ ID NO .: 21. Provided are a glycosyltransferase， a mutant， and an in vitro glycosylation method. The method comprises the steps of: transferring a glycosyl group of a glycosyl donor to a hydroxyl group at the C-3 position of a tetracyclic triterpenoid compound in the presence of a glycosyltransferase to form a glycosylated tetracyclic triterpenoid compound， wherein the glycosyltransferase is a glycosyltransferase as shown in SEQ ID NO.:4 or a polypeptide derived therefrom， or a glycosyltransferase as shown in SEQ ID NO.:21 or a polypeptide derived therefrom. Provided are a glycosyltransferase， a mutant， and an in vitro glycosylation method. The method comprises the steps of: transferring a glycosyl group of a glycosyl donor to a hydroxyl group at the C-3 position of a tetracyclic triterpenoid compound in the presence of a glycosyltransferase to form a glycosylated tetracyclic triterpenoid compound， wherein the glycosyltransferase is a glycosyltransferase as shown in SEQ ID NO.:4 or a polypeptide derived therefrom， or a glycosyltransferase as shown in SEQ ID NO.:21 or a polypeptide derived therefrom.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
