Supplementary information files for Cell culture-derived extracellular vesicles: Considerations for reporting cell culturing parameters

Supplementary files for article Cell culture-derived extracellular vesicles: Considerations for reporting cell culturing parametersCell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM‐derived EVs (CCM‐EV). The CCM‐EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell‐specific recommendations may need to be established for non‐mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM‐EV research.

补充文件：细胞培养来源的细胞外囊泡：细胞培养参数报告的考量
细胞培养条件培养基（CCM）作为基本科学、治疗和诊断应用中细胞外囊泡（EVs）的重要来源，具有极高的价值。细胞培养参数对CCM来源的细胞外囊泡（CCM-EVs）的生化组成、释放以及可能的生物学功能产生影响。国际细胞外囊泡学会严谨与标准化委员会的CCM-EVs工作小组致力于识别相关的细胞培养参数，基于现有知识描述其影响，推荐报告参数，并确定待解决问题。虽然一些建议适用于所有细胞类型，但对于非哺乳动物来源，如细菌、酵母和植物细胞，可能需要制定特定的细胞特异性建议。本文从视角论文中总结了实现这些目标目前的进展，并附有清单，以促进细胞培养参数的透明报告，从而提高CCM-EVs研究的可重复性。