Equid herpesvirus type 1 generates factor Xa (FXa) in a dose-dependent manner.
收藏Figshare2015-12-03 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Equid_herpesvirus_type_1_generates_factor_Xa_FXa_in_a_dose_dependent_manner_/1392216
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Factor Xa generation (nM) was measured with purified virus at different plaque forming units/cell (PFU/cell) using a one stage amidolytic assay with exogenous human FVIIa (1 nM) and FX (75 nM) and a chromogenic FXa substrate (n = 4). The FXa concentration was derived from comparing the rate of substrate cleavage in the sample (Vmax) to a standard curve of serially diluted purified human FXa. Factor Xa generation was below the linearity of the assay (0.17 nM FXa) in negative controls (samples without added FVIIa or FX or RK lysate with all reagents).* Below assay linearity.Equid herpesvirus type 1 generates factor Xa (FXa) in a dose-dependent manner.
创建时间：
2015-12-03
