Direct sulphate-TOR signalling control transcription reprogramming for shoot apex activation in Arabidopsis

Method: The 7-day sulphur starvation Col-0 seedlings were recovered by 2 h treatment of 10 uM sulphate, 10 uM Cys or 10 uM GSH with or without pretreated Torin2 (25 uM) for 1 h. Total RNA was isolated from shoot apex of seedlings. Three biological repeated were prepared.Purpose: Examine sulphate-, Cys- and GSH-regulated transcriptomes in Arabidopsis shoot apex.Abstract: Sulphur is an essential nutrient for plants that could be incorporated into sulphur-containing amino acids, such as Cysteine and GSH to sustain growth, development and stress adaptation. Little is known about sulphur sensing signalling in plants, mainly hindered by the innate capacity of plant cells to produce myriad complexity of sulphur-containing amino acids and compounds via sulphur assimilation pathway. To better understand the molecular landscape of the sulphate-TOR signalling network in shoot apex activation, we performed RNA-seq analyses to compare transcriptome changes specifically in shoot apex trigger by 2 h treatment of 10 uM sulphate, 10 uM Cys or 10 uM GSH in sulphate-starved seedlings with or without Torin2 supply.