DataSheet1_SYDE1 Acts as an Oncogene in Glioma and has Diagnostic and Prognostic Values.docx

Objectives: Gliomas remain one of serious public health problems worldwide which demand further and deeper investigation. The aim of this study was to explore the association between synapse defective protein 1 homolog 1 (SYDE1) and gliomas via public database analysis and in vitro validation to determine the potential diagnostic and prognostic values.Methods and Results: Compared with healthy brain tissues, there was a significant increase in SYDE1 expression in glioma tissues. Additionally, SYDE1 exhibited higher expression levels in glioma patients with unfavorable clinicopathological factors. In vitro knockdown of SYDE1 in glioma cell lines A172 inhibited their migrative and invasive ability but not the proliferative ability. GO and KEGG pathway analysis of the top 100 genes coexpressed with SYDE1 showed enrichments of tumor-associated terms. Further bioinformatic analysis revealed that the SNHG16/hsa-miR-520e/SYDE1 axis might be involved in glioma development.Conclusions:SYDE1 is expressed at higher levels in gliomas than in healthy brains, and can promote metastasis and invasion but not proliferation of gliomas. Furthermore, SYDE1 has values in the diagnosis and prognosis prediction of gliomas.

研究目标：胶质瘤是全球范围内严重的公共卫生问题之一，亟需进一步深入研究。本研究旨在通过公共数据库分析和体外验证，探讨突触缺陷蛋白1同源物1（SYDE1）与胶质瘤之间的关联，以确定其在诊断和预后评估中的潜在价值。研究方法与结果：与健康脑组织相比，胶质瘤组织中SYDE1的表达显著增加。此外，具有不良临床病理因素的胶质瘤患者中，SYDE1的表达水平更高。在体外敲低胶质瘤细胞系A172中的SYDE1，可以抑制其迁移和侵袭能力，但不会影响其增殖能力。对与SYDE1共表达的前100个基因进行的GO和KEGG通路分析显示，肿瘤相关术语富集。进一步的生物信息学分析揭示，SNHG16/hsa-miR-520e/SYDE1轴可能与胶质瘤的发生发展有关。研究结论：与正常大脑相比，胶质瘤中SYDE1的表达水平更高，可促进胶质瘤的转移和侵袭，而非增殖。此外，SYDE1在胶质瘤的诊断和预后预测方面具有重要价值。