Data underlying the publication: MOSAIC: a highly efficient, one-step recombineering approach to plasmid editing and diversification

Data underlying the publication: van den Brink, M., Althuis, T. Y., Danelon, C. &amp; Claassens, N. J. MOSAIC: A Highly Efficient, One-Step Recombineering Approach to Plasmid Editing and Diversification. <em>ACS Synthetic Biology</em> <strong>14</strong>, 3880–3889 (2025). DOI: 10.1021/acssynbio.4c00657.<br>This dataset contains data collected during experiments as part of Marijn van den Brink's and Timotheus Althuis' PhD projects. The raw data consists of Nanopore sequencing data. Nanopore sequencing was performed by Plasmidsaurus (Oregon, US). The FASTQ files were then mapped to reference sequences, resulting in .bam/.bai files. These were further analyzed using an in-house developed R script. All data processing and analysis steps are described in detail in the methods section of the publication.<br>The data is grouped in folders based on the figures in the publication. The folder name specifies the figure number and contains a short description of the data. Each folder contains raw data (FASTQ) and processed data. The processed data consists of (i) the mapped reads (file format: .bam and .bai), (ii) the analysis results of the mapped reads (file format: .csv and .xlsx), (iii) the graphs plotting the results (file format: .pzfx), and (iv) the R scripts used to produce the results incl. the required .xlsx or .csv files.<br>To analyze your own data using our R script, go to "General Nanopore data analysis scripts &gt; MOSAIC-library-analysis-script.R". This R script contains information on how to adapt the script to your own data.

本数据集关联的已发表论文：van den Brink, M.、Althuis, T. Y.、Danelon, C. 与 Claassens, N. J. 《MOSAIC：一种高效的一步重组工程质粒编辑与多样化方法》，*ACS合成生物学* **14**卷，第3880–3889页（2025年）。DOI: 10.1021/acssynbio.4c00657.
本数据集包含Marijn van den Brink与Timotheus Althuis博士研究项目中实验采集的数据。原始数据为纳米孔（Nanopore）测序数据，测序工作由美国俄勒冈州的Plasmidsaurus公司完成。随后将FASTQ文件比对至参考序列，生成.bam与.bai格式文件，再通过自研R脚本开展进一步分析。所有数据处理与分析步骤详见该论文的方法部分。
本数据集按照论文中的图表分组存储于不同文件夹，文件夹名称标注了对应图表编号，并附带数据的简短说明。每个文件夹内均包含原始数据（FASTQ格式）与处理后数据：(i) 比对后的测序读段（文件格式：.bam与.bai）；(ii) 比对读段的分析结果（文件格式：.csv与.xlsx）；(iii) 结果绘图文件（文件格式：.pzfx）；(iv) 用于生成分析结果的R脚本，以及所需的.xlsx或.csv辅助文件。
若需使用本研究的R脚本分析您的自有数据，请前往「通用纳米孔数据分析脚本 > MOSAIC-library-analysis-script.R」。该R脚本中包含针对您的自有数据调整脚本的相关说明。