Chromophore-driven optical path reduction as an indirect inner filter effect correction strategy in fluorescence microplate assays - experimental data

Experimental data for the paper entitled Chromophore-driven optical path reduction as an indirect inner filter effect correction strategy in fluorescence microplate assays (manuscript in preparation; citation data will be updated pending publication). 

An Excel file with multiple worksheets is provided for the following:

1.    Fluorescence data:

The worksheets contain fluorescence values measured in tetraplicates using two microplate readers (Tecan Spark M10, Tecan, Austria and SpectraMax iD3, Molecular Devices, USA), with baseline values recorded in octuplicates. The results were averaged and baseline-corrected. Detailed notes on the titration experiments are included in the manuscript. Data include both IFE-uncorrected fluorescence and fluorescence corrected (fully or partially) by the addition of an absorbing chromophore.

2.    Absorbance data:

All measured absorbance values obtained using the Tecan Spark M10 microplate reader with UV-Vis transparent microplates are included. The absorbance values at specific wavelengths suitable for the Lakowicz IFE correction method or for determining the concentration of the compound are given separately.

3.    Fluorescein spectra:

UV-Vis and fluorescence excitation/emission spectra for highly dilute fluorescein concentrations where the inner filter effect (IFE) is negligible are reported. These measurements were performed to obtain “true” excitation and emission spectra without the influence of IFE quenching effects.

4.    ZINFE/NINFE IFE corrections:

The dataset contains the results of ZINFE/NINFE IFE corrections (https://doi.org/10.1021/acs.analchem.2c01031) applied to the fluorescence data and processed using the web service available at https://ninfe.science.

The notation of the fluorophores and microplates is the same as in the manuscript. For reasons of visual clarity, the numerical titration indices are not in subscript.