Validation of the algorithm output.

Two WISH experts marked each of 200 processed images as good, intermediate or bad (1.0, 0.5 or 0.0) on three potentially subjective qualities: whether the embryo outline had been captured correctly, whether the stained regions were delineated correctly, and whether the pigmented regions were delineated correctly. For each quality we correlated the experts’ scores over all 200 images.