Gene expression in Peyronie disease, tunica albuginea, and corpora cavernosum in cultured cells from human penis

Cells from normal tissues and from a Peyronie Disease plaque of human penis were cultured and RNA was extracted. The aim was to investigate differences in gene expression in the abnormal plaque as well as to establish baseline expression data in normal tissues for comparison. Cells were incubated in six well plates in the presence or absence of TGFb, a known inducer of Peyronie Disease-like plaque in animal systems. RNA was extracted using Trizol reagent and the RNA was further purified for DNA microarray analysis using the Qiagen RNeasy Plus Micro kit. RNA samples were analyzed by the UCLA DNA Core using the Affymetrix human Gene 1.0 ST system. Data were normalized by the UCLA core.本研究对人类正常阴茎组织与佩罗尼病（Peyronie Disease）斑块来源的细胞进行体外培养并提取RNA，旨在探究异常斑块内的基因表达差异，并建立正常组织的基因表达基线数据以用于对照比较。将细胞接种于六孔板中，分别加入或不加入转化生长因子β（TGFb）——该因子在动物模型中已被证实可诱导出佩罗尼病样斑块。采用TRIzol试剂提取RNA，随后使用凯杰（Qiagen）RNeasy Plus Micro试剂盒对RNA进行纯化，以用于DNA微阵列分析。RNA样本由加州大学洛杉矶分校（UCLA）DNA核心实验室采用Affymetrix人类基因1.0 ST芯片系统完成检测。所有数据均由该核心实验室进行标准化处理。

搜集汇总

背景与挑战

背景概述

该数据集通过微阵列技术分析了人类阴茎正常组织（白膜和海绵体）与佩罗尼病斑块在培养细胞中的基因表达，重点关注TGFb刺激下的差异。研究旨在建立正常组织的基线表达数据，并探究疾病斑块的异常基因表达模式，为佩罗尼病的机制研究提供基础。数据集包含6个样本，使用标准化Affymetrix平台，数据于2017年公开。