LC-MS/MS sequenced proteins extracted from Stylophora pistillata skeleton; analyzed in the Falkowski lab at Rutgers from 2010-2014 (CROA project)

<p><em>This dataset includes information presented in Supplemental Table S2 from Drake et al. 2013:</em><br />
Table S2. Putative homologous proteins from other mineralizers or related organisms. The most similar predicted protein sequence from each comparison organism is given. Lack of a similar protein sequence for a given species is noted as “–”.<br />
See the <a href=\"http://dmoserv3.whoi.edu/data_docs/CROA/SI_T1.xlsx\" target=\"_blank\">original Excel file</a> for the complete sequences of the most similar predicted protein sequence from each comparison organism.</p>

<p>Refer to the following publication for more information:<br />
Drake, J. L., T. Mass, L. Haramaty, E. Zelzion, D. Bhattacharya &amp; P. G. Falkowski. 2013. Proteomic analysis of skeletal organic matrix from the stony coral <em>Stylophora pistillata</em>. Proceedings of the National Academy of Sciences 110(10): 3788-3793. doi: <a href=\"http://dx.doi.org/10.1073/pnas.1301419110\" target=\"_blank\">10.1073/pnas.1301419110</a></p>

<p>Also refer to:<br />
<a href=\"http://dmoserv3.whoi.edu/data_docs/CROA/SI_F2_revise.pdf\" target=\"_blank\">Figure S2. Predicted amino acid sequences of 36 S. pistillata proteins (PDF)</a>. Peptides detected by LC-MS/MS after tryptic digestion are in bold and after proteinaseK digestion are underlined. Translations of internal sequences confirmed by PCR amplification of <em>S. pistillata</em> cDNA using gene-specific primers are highlighted in gray. Discrepancies between the predicted sequence and that determined by translation of PCR product are in red. The secretion signal peptide of P12, is crossed out over the portion that is predicted to be cleaved before secretion.<br />
<a href=\"http://dmoserv3.whoi.edu/data_docs/CROA/SI_T1_Primer_sets.pdf\" target=\"_blank\">Table S1. SOM protein primer sets (PDF)</a>. Gene-specific primers used to confirm the DNA and cDNA sequences of selected SOM proteins.</p>