Characterization of patterns of somatic alterations between matched primary and metastatic colorectal tumors using whole genome sequencing technology

We performed whole genome sequencing analysis on two primary tumors and their liver metastases. By comparing to matched germline DNA, we systematically identified somatic alterations in the primary tumors and their matched metastatic tumors. We also found distinct clonal evolution patterns between primary and metastatic tumors in the two cases studied here.