Bioinformatic and Laboratory Evidence for a Functional Rickettsiales vir Homolog (rvh) T4SS.

1Vir and Vir-like proteins, following the nomenclature of the A. tumefaciens vir system.2Percentage of 62 bacterial T4SSs that contain each Vir or Vir-like protein [29].3Rickettsiales virhomolog. Proteins previously not annotated as Vir via consensus annotation are noted with an asterisk.4Average size of the Rickettsia orthologs across 13 genome sequences. Numbers for RvhB6a-e show only the VirB6/TrbL domains; avg, lengths of full sized RvhB6 proteins are as follows: RvhB6a = 1055 aa; RvhB6b = 674 aa; RvhB6c = 971 aa; RvhB6d = 886 aa; RvhB6e = 1154 aa.5Laboratory studies demonstrating putative function. A = gene expression analysis of R. conorii[213]; B = gene expression analysis of R. prowazekii[214]; C = gene expression analysis of R. typhi[215]; D = bacterial two-hybrid screen of R. sibirica[74]; E = proteome analysis of R. felis[216]; Fa = PhoA fusion analysis of R. typhi[99]; Fb = gene expression analysis of PhoA fusion positive proteins of R. typhi[99]; G = proteome analysis of R. prowazekii str. Madrd E [217]; H = Differential translation analysis between R. prowazekii strains Madrid E and Breinl [218].