Benthic microbial communities of Fram Strait
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB34672
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Using TV-guided multicorer, sediment samples were collected in Fram Strait during the Polarstern cruise PS99.2 (June 24th – July 16th 2016) from Greenland shelf to west coast of Spitsbergen. For assessing bacterial and archaeal community composition, a slurry of subsampled uppermost centimeter of each core were filtered with peristaltic pump through 0.22 μm membrane. The extracted genomic DNA was isolated and the hypervariable V4-V5 (515F - 926R) region of the 16S rRNA was amplified and sequenced using Illumina MiSeq platform in a 2 × 300 bp paired-end run.
创建时间:
2021-09-13



