Investigated the responses of ZM532 and wild-type ZM4 to acetic acid and furfural by using RNA-seq

In our previous study, we successfully constructed an engineered Zymomonas mobilis ZM532 strain tolerant these double inhibitors by genome shuffling, but the molecular mechanisms of tolerance to these inhibitors are still unknown. The goal of this study investigated the responses of ZM532 and wild-type ZM4 to acetic acid and furfural using Transcriptome Overall design: ZM532 and wild-type ZM4 cultured in RM and AF medium in triplicate and then extracted the RNA. The concentrations of acetic acid and furfural combinations were set at 5.0 g/L and 3.0 g/L to study the responses of ZM4 and mutant ZM532.