RNA-seq gene expression of flow cytometry sorted breast cancer stem cell populations

MMTV-PyMT transgenic FVBN mice spontaneously developed breast tumors. Two tumors were collected as replicate samples, and cells from the dissociated tissue were sorted into 2 cancer stem cell (CSC) populations, along with the remaining non-CSCs. The samples were submitted for RNAseq to generate differential gene expression profiles of the breast tumor CSC populations. Overall design: Gene expression profile of ALDH positive and CD29 hi, CD61 positive CSC populations compared to non-CSC from mouse breast tumor.