Single cell RNA sequencing of distal lund epithelial cells generated from human induced pluripotent stem cells. Single cell RNA sequencing of distal lund epithelial cells generated from human induced pluripotent stem cells

We perform transcriptomic profiling of a directed differentiation protocol for generating distal lung epithelial cells and alveolar type II epithelial cells (AEC2s) from pluripotent stem cells (PSCs) using bi-fluorescently targeted iPSC cell line BU3-NGST. On day 33 of distal lung differentiation, we sorted SFTPCtdTomato/NKX2-1GFP double positive cells and NKX2-1GFP single positive cells for Fluidigm single cell RNA sequencing analysis. We find canonical AEC2s genes expressed in both sorted populations but at a higher level in the SFTPCtdTomato/NKX2-1GFP double positive population. We also find genes associated with cellular proliferation to be enriched in the NKX2-1GFP single positive population, suggestive of a less mature but more proliferative subset of PSC-derived AEC2s. Overall design: Global single cell transcriptomic analysis of day 33 PSC-derived distal lung epithelial cell populations identified by NKX2-1GFP and SFTPCtdTomato reporters.