A novel zebrafish model of intestinal epithelial damage reveals macrophages and igfbp1a as major modulators of mucosal healing

To elucidate the cellular and molecular mechanisms governing intestinal damage and regeneration in zebrafish, we performed single-cell (sc)RNA-seq from dissected intestines. Overall design: Intestines from control and Mtz-treated zebrafish larvae were dissected at 4 days post fertilization (dpf) and 5dpf. Single cell suspensions from dissected intestines were prepared througn enzymatic and mechanical disaggregation, and DAPI- live cells were sorted by FACS.