Microarray gene expression profile of primary breast tumour tissue. Microarray gene expression profile of primary breast tumour tissue

Data were used for study of changes in gene expressions in breast primary tumour of patients with circulating tumour cells positive on mesenchymal marker (CTC EMT). Study aimed to identify signaling pathways associated with the presence of CTC_EMT in PBC patients. This translational study included 17 patients with PBC and 5 donors of normal breast tissue. CTC_EMT were detected before surgery by quantitative RT-PCR assay for expression of epithelial-to-mesenchymal transition (EMT) genes (TWIST1, SNAIL1, SLUG, ZEB1). Total RNA was extracted, in parallel, from the fresh frozen primary tumor and whole-transcriptome profiles were obtained using RNA sequencing and by microarray. RNA-seqquencing version of same samples has been submitted to SRA (BioSaples accessions are provided here). Also data under BioProject PRJNA751534 originate from the same samples. Overall design: 17 patients with primary breast cancer (PBC) tumour tissue and 5 donors of normal breast tissue