Single-cell behavioral phenotyping reveals calcium-dependent coordination of bacterial motility systems

This deposit contains all of the processed data and analysis scripts supporting the findings in “Single-cell behavioral phenotyping reveals calcium-dependent coordination of bacterial motility systems.” /Data.zip Contains all processed cells trajectories and focal-adhesion datasets used to generate figures and quantitative analyses in the manuscript. /Cells_data Agar_0p75: pickle files with per‐cell, per‐frame (x,y) trajectories for leading pole, lagging pole, and center on 0.75 % agar. Ibidi_chitosan: same format for trajectories recorded on chitosan-coated coverslips. Umap: two-dimensional embeddings of the 48 motility features for all strains. /FACS_data Agar_predation: detected focal-adhesion complexes (FACS) from AglZ-YFP in predation assays; one .pkl per time‐lapse. Ibidi_chitosan: FACS spot coordinates for both A⁺S⁻ and WT strains on chitosan substrates. /Scripts.zip Includes all code and configuration files required to reproduce data processing, analysis, and figure generation: config/parameters.jsonDefines data paths and processing and analysis settings. modules/Python modules implementing image preprocessing, segmentation, tracking, feature extraction (kymographs, pole detection), FACS detection, and trajectory feature computation. run_pipeline.pyOrchestrates raw data ingestion, processing through most modules for cell tracking, and exports the final .pkl datasets.