Single-cell RNA sequencing Reveals Obesity-Induced Inhibition of Testosterone Production and Damage of Blood-Testis Barrier Integrity

Obesity is accompanied by multiple known health risks and increased morbidity, and the prevalence of obesity continues to rise. Fertility in males of reproductive age is affected by the endocrine abnormalities and comorbidities associated with obesity, which has become a primary cause of male infertility. Single-cell RNA sequencing (scRNA-seq) of testes in obese individuals has not been done, so we chose to use this technique to study the mechanism of infertility in an obese mouse model. Overall design: Male ICR mice were randomly divided into a conventional diet (CD) group and a high-fat diet (HFD) group at the age of 6 weeks. Testes of the two groups were collected and analyzed by scRNA-seq.