MicroRNA expression levels in plasma exosomes from liver cancer patients before and after surgery [miRNA-Seq]

Exosomes play a crucial role in the occurrence and development of tumors. To investigate the levels of different exosome expressions in the plasma of liver cancer patients before and after liver tumor resection, we conducted high-throughput sequencing of miRNA in preoperative and postoperative blood exosomes from liver cancer patients. Blood samples were collected from liver cancer patients before and after surgery. Exosomes were isolated using ultracentrifugation, and RNA was extracted using an RNA extraction kit to extract microRNA from the exosomes. Subsequently, high-throughput sequencing was performed on the isolated microRNA.