(TMT)-quantitative proteomic study of Bacillus cereus tsu1

In this project, we report the bacterial proteome expression profile change for different time points when culturing in rapeseed cake substrate (RCS). Bacillus cereus tsu1 was cultured in RCS (25g/L) for 12 hours, 24 hours and 48 hours. At each time point, cells were stained with Sudan Black to monitor PHB accumulation. Bacterial cells were loaded with PHB after 12-hour culture and significant degradation of PHB was observed after 48-hour culture. Bacterial proteome profile changes were identified using tandem mass tags mass spectrometry (TMT-MS)-based quantitative proteomics analysis. From TMT proteomics analysis, 3,237 proteins were detected and quantified from the bacterial pellet protein extraction, from which proteins related with PHB biosynthesis and degradation were identified. Quantitative analysis revealed that 146 (between 12h and 24h samples), and 158 (between 12h and 48h) proteins showed significant differences. Several STRING protein interaction networks were developed for significantly changed protein related with sporulation and anaerobic respiration.