Sporozoite Proteome (IowaII)

C. parvum sporozoite peptides from 2D gel LC-MS/MS analysis. Purified oocysts of Cryptosporidium parvum IowaII passaged in lambs were excysted. Freshly excysted sporozoites were solubolized and protein preparations were analyzed by 2 dimensional electrophoresis LC-MS/MS. Spectra were searched against a Cryptosporidium database using SEQUEST. SEQUEST results were assembled and filtered using the DTASelect (version 2.0) program.