Gonadal gap junctions activate Notch signaling to suppress intestinal innate immunity via p38/PMK-1 and lysosome pathway

We showed that the innexin genes encoding gap junction subunits positively regulated GLP-1/Notch signaling to suppress the innate immunity of C. elegans via PMK-1/p38 pathway. The unc-17 acts in the dowmstream of inx-14/glp-1 to regulate innate immunity. Transferring synchronized larval 4-stage wild type N2 and unc-17(e245) worms to a new plate with PA14 infection or OP50 fed and collecting worm samples after 24 hours. Then design some different assays to identify the important tips obtained from RNA-seq data analysis.