Contrast reference images for the Timm-Haug73 modification of Timm's sulphide silver method (v1)

The dataset comprises high-resolution bright-field microscopy images of serial horizontal sections from the half-brain of one adult Wistar rat. The sections were stained with the Timm-Haug73 modification of Timm’s method, which visualises zincergic terminal fields and metal-containing glia. Contrast and the visibility of regional and cytological features increase with section incubation time in the developer solution, and the optimal developing time varies between regions and different cytological features The dataset contains images of 5 series of sections developed with incremental incubation times, increased in 10-minute steps from 30 to 70 minutes. The set of series was used as a rough benchmark for “staining strength” in the Timm-Haug73 procedures, to create the moderately (Timm-light) and strongly (Timm-dark) stained series of the dataset "Multiplane microscopic atlas of rat brain zincergic terminal fields and metal-containing glia stained with Timm’s sulphide silver method".