HopZ1a interacts with the soybean protein GmJAZ1.

(A) HopZ1a and GmJAZ1 interact in vitro. GmJAZ1-HA and GST-HopZ1a proteins were expressed in E. coli. Precipitation of GmJAZ1 with HopZ1a was determined by western blots before (Input) and after affinity purification (Pull-down) using anti-HA antibody. The protein abundances of GST, GST-HopZ1a and GST-HopZ1a(C216A) on the affinity resins after washes were detected by Coomassie blue staining. (B) Bimolecular fluorescence complementation analysis showing HopZ1a-GmJAZ1 interactions in plant cells. HopZ1a(C216A)-nYFP and GmJAZ1-cYFP were co-expressed in N. benthamiana using Agrobacterium-mediated transient expression. Leaves co-infiltrated with Agrobacterium carrying GmJAZ1-cYFP+nYFP or cYFP+HopZ1a(C216A)-nYFP were used as negative controls. Fluorescence was detected by confocal microscopy from the infiltrated tissues at 48 hpi. DAPI was used to stain the nuclei. These experiments were repeated three times with similar results.