Hypothermia Affects Translocation of Numerous Cytoplasmic Proteins Following Global Cerebral Ischemia

Using a decapitation ischemia model, we studied translocation of proteins to and from the cytosol in
normothermic (NT) and hypothermic (HT) rat brains. 2D gel analysis identified 74 proteins whose
cytosolic level changed significantly after 15 min of ischemia. HT preserved the cytosolic levels of several
glycolytic enzymes, as well as many plasticity related proteins, otherwise decreased following NT
ischemia. The levels of redox-related proteins was lower in HT than in NT. Our results indicate that
translocation of proteins to and from the cytosol is an important issue during ischemia.
Keywords: 2D gel • cortex • cytoskeleton • glycolysis • LC−MS/MS • MALDI • plasticity • redox-sensitive