Raw dataset for bacterial exopolysaccharide production using cassava pulp as substrate by Bacillus spp. isolated from Thai milk kefir with its antioxidant and antimicrobial capacities

This raw dataset reflects an analysis of the antioxidant and antibacterial activity of exopolysaccharide (EPS) produced by eight Bacillus spp. isolated from Thai milk kefir [1] on a substrate of cassava pulp. Cassava pulp was analyzed for its moisture, ash, fat, protein, fiber, starch and sugar, neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL) when used as an EPS-producing substrate. After 3 days of fermentation, dataset on the total plate count, the reducing sugar content, the pH of the culture, and the fresh and dry weight of the EPS was recorded. In response to the EPS produced by several Bacillus spp., the total phenolic content, total flavonoid content, antimicrobial activity against Streptococcus agalactiae and Staphylococcus aureus, and antioxidant activities assessed by DPPH scavenging assay, FRAP assay and hydroxyl radical scavenging activity were collected. Agar disc diffusion, spectrophotometry for antioxidant activity and bioactive content, proximate analysis, the dinitrosalicylic acid method, pH meter readings, the total plate count via serial dilutions using the spread plate technique were the primary techniques used.