Resequencing of K. phaffii platform strains for comparison of ARS based expression clones. Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Resequencing of two platform strains based on K. phaffii BSYBG11 (bisy GmbH, Hofstätten/Raab, Austria). One sample, BSYBG11gut1_int-ref, is an average CalB production clone based on strain harboring a gut1 deletion and integration of plasmid pBSY3G_DaCalB_PAgTEF1. The other one, BSYBG11tpi1, only carries a clean deletion of tpi1. Both strains were cultured over night. Cell pellets were sent to Macrogen (Macrogen Inc., Seoul, South Korea) für gDNA extraction and sequencing. Both libraries were prepared using TruSeq PCR free kit, target insert size of 550 and were sequenced on a NovaSeq 6000 as 150 bp pairedßend reads.