Unusual enzymology and peptide natural products from novel Acidobacteriota spp. associated with reef-building corals

Unusual enzymology and peptide natural products from novel Acidobacteriota spp. associated with reef-building corals Raw Data for characterisation of RiPP BGCs from Acidobacteriota: Plasmids Plasmid maps of constructs used for heterologous production of precursors and maturases in E. coli in .gb format. Aci .raw LC-MS files of proteolytic digests (GluC) of AciA1 and AciA2 produced with or without AciKC. Tha .raw LC-MS files of proteolytic digests (Try or AspN) of ThaA produced with or without ThaM and/or ThaO.Subfolders include LC-MS files of 13C and 15N labelling data, derivatisation with IAA, RaneyNi, and Marfey's reagent, and point mutants. The .raw LC-MS files of proteolytic digests (AspN) of native TheA produced with or without TheO/ TheB/ TheM/ TheN, as well as LC-MS files of proteolytic digests (AspN or Try) of point mutants, truncations and cargo-fusions produced in the presence of TheO or without.Additional subfolders include MS/MS data and plasmids as .gb files. NMR Raw data files for 1H (1), 1H-1H COSY (2), NOESY (3), HSQC (4), and HMBC (5) spectra of purified and modified tryptic fragment of ThaA. MolecularNetworking Output files generated from GNPS (one folder for the pellet network and one for the supernatant network). CrudeExtracts .raw LC-MS files of crude extracts from S. corallicola supernatant (SN) or pellet (P) cultured with and without coral-derived dissolved organic matter. Extracts from medium with or without DOM (but no S. corallicola) are labelled with "_neg". RNAseq R script and input files--including raw feature counts, plasmidsaurus feature annotations, antiSMASH feature annotations and DESeq2 results--used to analyse BGC transciption data from cultivating S. corallicola with and without coral-dervied dissolved organic matter. CellCounts Raw data files, metadata, and modified cell count data from cultivating S. corallicola with and without coral-dervied dissolved organic matter.