snRNAseq of humanized mouse model of late-onset Alzheimer's Disease

Mice were bred such that they are homozygous for human MAPT (tau), and one of two versions of the APP gene (NL and NLF) referring to the Swedish familial Alazheimer's Disease (AD) variants, and one of human APOE allele E3 or APOE allele E4. APP-NLF+APOE(E3) mice develop amyloid plaques and attention and memory deficits at 16-24 months, whereas APP-NLF+APOE(E4) mice develop this phenotype earlier at 9 months. Three mice of each of the four possible genotypes were sacrificed at 9 months of age, and the prefrontal cortex was dissected and subject to single nucleus RNA sequencing (snRNAseq) with the 10X Chromium platform.