Figure S1 - Implication of TLR- but Not of NOD2-Signaling Pathways in Dendritic Cell Activation by Group B <i>Streptococcus</i> Serotypes III and V

Cytokine response of knock-out cells using specific TLR-ligands. Control (CTRL), MyD88-/-, TLR2-/- or NOD2-/- bone marrow-derived dendritic cells (DCs) were stimulated with ultra-pure lipopolysaccharide (LPS, 1 µg/ml), PAM(3)CSK(4) (0.5 µg/ml) or muramyl dipeptide (MDP, at 10 µg/ml). Non-stimulated cells served as negative control (C-) for basal expression levels. Supernatants were harvested at 16 h of incubation and cytokine production quantified by ELISA. Data are expressed as mean ± SEM (in ng/ml) from four independent experiments. ** P<0.01, indicates statistically significant differences between CTRL and knock-out cells at the same condition. (EPS)