Final qPCR designs for the reference genes and the genes of interest (GOIs).

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Primer and probe sequences, the flourophores and quenchers used for the TaqMan probes and the amplicon length (in base pairs (bp)) of the product for each gene are listed. Reference genes GUSB (NM_001003191) and PSMB6 (XM_844148) were designed in a duplex assay. GOIs Ki-67 (XM_005637893), CAIX (NM_001145174) and GLUT3 (NM_0010003308) were designed in simplex assays; GOIs HIF-1α (XM_003639201), TF (NM_001024640) and VEGFA (NM_0010003175) in a triplex assay and GOIs HK2 (XM_003639587) and GLUT1 (NM_001159326) in a duplex assay. Final qPCR designs for the reference genes and the genes of interest (GOIs).

创建时间：

2015-10-26