RNA-seq for splenic gene expression in response to Newcastle disease virus challenge in two chicken lines with different disease resistance

In order to have a better understanding about gene expression response to NDV in chicken spleen, and also to unravel genetic regulation related to resistance to NDV, gene expression in spleen of two chicken lines [Fayoumi (resistant line) and Leghorn (susceptible line)] with different resistance to infectious diseases were investigated. Each line was divided into two groups (3-4 chickens/group) that are respectively treated by NDV(200 ul of 107 EID50%) and Phosphate-buffered saline (PBS) through nasal and ocular inoculation routes at 21 days post hatch. Gene expression in spleen was then detected by RNA-seq at 2 and 6 day post inoculation (dpi).