Mapping of SiWSL1 of foxtail millet using BSA-seq through the mutant white stripe leaf 1 (wsl1)

The mutant white stripe leaf 1 (wsl1) was identified from a spontaneous mutant derived from Changnong 35, a foxtail millet cultivar. BSA-seq was conducted for mapping the mutant gene, an F2 population derived from the cross wsl1 and Pinzi39 was selected to construct DNA pools, and two DNA pools, namely green pool (G pool) and white-striped pool (W pool), consisted of an equal amount of DNA from 50 F2 individuals showing the most extreme leaf green and white-striped phenotype, respectively, and then wsl1, Pinzi39, G pool and W pool were sequenced on an Illumina HiSeq system using the standard protocol.