Conservative aa substitutions occurring during long-term replication of genome-length HCV RNAs (II).

aConservative aa substitutions detected in at least two of four cell line series. bConservative aa substitutions detected in HuH-7-derived cell line series (O, OA, OB, OD, or OE) used in the previous study [20]. cConservative aa substitutions that became the same aa as JFH-1strain. Conservative aa substitutions detected after 2-year and 4-year cultures are shown by bold letters. Conservative aa substitutions detected only after 2-year culture are shown in parentheses.